Variegatic acid from the edible mushroom Tylopilus ballouii inhibits TNF-α production and PKCβ1 activity in leukemia cells

Kouichi Sugaya; Mutsumi Ino; Naoki Matsuo; Jun-Ichi Onose; Naoki Abe

doi:10.1016/j.bmcl.2019.126886

Variegatic acid from the edible mushroom Tylopilus ballouii inhibits TNF-α production and PKCβ1 activity in leukemia cells

Bioorg Med Chem Lett. 2020 Feb 15;30(4):126886. doi: 10.1016/j.bmcl.2019.126886. Epub 2019 Dec 13.

Authors

Kouichi Sugaya¹, Mutsumi Ino¹, Naoki Matsuo¹, Jun-Ichi Onose², Naoki Abe³

Affiliations

¹ Department of Nutritional Science and Food Safety, Faculty of Applied Bio-Science, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
² Department of Nutritional Science and Food Safety, Faculty of Applied Bio-Science, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan. Electronic address: j1onose@nodai.ac.jp.
³ Department of Nutritional Science and Food Safety, Faculty of Applied Bio-Science, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan. Electronic address: abe@nodai.ac.jp.

PMID: 31879206
DOI: 10.1016/j.bmcl.2019.126886

Abstract

Variegatic acid, isolated from Tylopilus ballouii dry fruiting bodies, is an inhibitor of β-hexosaminidase release and tumor necrosis factor (TNF)-α secretion from rat basophilic leukemia (RBL-2H3) cells, with IC₅₀ values of 10.4 μM and 16.8 μM, respectively. On the other hand, it inhibits PKCβ1 activity with an IC₅₀ value of 36.2 μM.

Keywords: Allergy; Chemokine; Inflammatory response; Variegatic acid.

MeSH terms

Animals
Basidiomycota / chemistry*
Basidiomycota / metabolism
Cell Line, Tumor
Inhibitory Concentration 50
Leukemia / metabolism
Leukemia / pathology
Mast Cells / cytology
Mast Cells / drug effects
Mast Cells / metabolism
Protein Kinase C beta / antagonists & inhibitors*
Protein Kinase C beta / metabolism
Rats
Staurosporine / pharmacology
Tumor Necrosis Factor-alpha / metabolism*

Substances

Tumor Necrosis Factor-alpha
Protein Kinase C beta
Staurosporine

Supplementary concepts

Tylopilus balloui